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c8d1a mouse cerebellar immortalized astrocytes  (ATCC)


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    ATCC c8d1a mouse cerebellar immortalized astrocytes
    In vitro characterization of Ten/TCAP gene expressions and TCAP-1 induced activation in mouse cerebellar astrocytes. Gene expression was determined using RT-PCR with RNA extracted from <t>C8D1A</t> mouse cerebellar astrocytes. (A) teneurins 1, 3 and 4 were expressed ( n = 4) and teneurin 2 was not expressed ( n = 4). TCAPs 1-4 were expressed (TCAP-1, n = 3; TCAP-2, n = 5; TCAP-3, n = 3; TCAP-4, n = 3). β-actin served as positive control ( n = 5). (B) fluo-4 fluorescence shown prior to 100 nM TCAP-1 administration (non-stimulated). (C) fluo-4 fluorescence shown 3 min after 100 nM TCAP-1 administration (TCAP-1 treatment). (D) differential interference contrast image (DIC) C8D1A astrocytes. Arrows are showing discreet band. (E) normalized fluo-4 fluorescence comparing vehicle (aCSF) and 100 nM TCAP-1 treated cells ( n = 3 for each treatment, where each n is an average of five cells per coverslip). Mean (± SEM) values from each group were submitted to two-way ANOVA and Bonferroni post hoc test. ** p < 0.01; *** p < 0.001.
    C8d1a Mouse Cerebellar Immortalized Astrocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c8d1a+mouse+cerebellar+immortalized+astrocytes/C8-D1A/pmc06609321-143-2-8
    Average 96 stars, based on 330 article reviews
    c8d1a mouse cerebellar immortalized astrocytes - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "A Putative Role of Teneurin-2 and Its Related Proteins in Astrocytes"

    Article Title: A Putative Role of Teneurin-2 and Its Related Proteins in Astrocytes

    Journal: Frontiers in Neuroscience

    doi: 10.3389/fnins.2019.00655

    In vitro characterization of Ten/TCAP gene expressions and TCAP-1 induced activation in mouse cerebellar astrocytes. Gene expression was determined using RT-PCR with RNA extracted from C8D1A mouse cerebellar astrocytes. (A) teneurins 1, 3 and 4 were expressed ( n = 4) and teneurin 2 was not expressed ( n = 4). TCAPs 1-4 were expressed (TCAP-1, n = 3; TCAP-2, n = 5; TCAP-3, n = 3; TCAP-4, n = 3). β-actin served as positive control ( n = 5). (B) fluo-4 fluorescence shown prior to 100 nM TCAP-1 administration (non-stimulated). (C) fluo-4 fluorescence shown 3 min after 100 nM TCAP-1 administration (TCAP-1 treatment). (D) differential interference contrast image (DIC) C8D1A astrocytes. Arrows are showing discreet band. (E) normalized fluo-4 fluorescence comparing vehicle (aCSF) and 100 nM TCAP-1 treated cells ( n = 3 for each treatment, where each n is an average of five cells per coverslip). Mean (± SEM) values from each group were submitted to two-way ANOVA and Bonferroni post hoc test. ** p < 0.01; *** p < 0.001.
    Figure Legend Snippet: In vitro characterization of Ten/TCAP gene expressions and TCAP-1 induced activation in mouse cerebellar astrocytes. Gene expression was determined using RT-PCR with RNA extracted from C8D1A mouse cerebellar astrocytes. (A) teneurins 1, 3 and 4 were expressed ( n = 4) and teneurin 2 was not expressed ( n = 4). TCAPs 1-4 were expressed (TCAP-1, n = 3; TCAP-2, n = 5; TCAP-3, n = 3; TCAP-4, n = 3). β-actin served as positive control ( n = 5). (B) fluo-4 fluorescence shown prior to 100 nM TCAP-1 administration (non-stimulated). (C) fluo-4 fluorescence shown 3 min after 100 nM TCAP-1 administration (TCAP-1 treatment). (D) differential interference contrast image (DIC) C8D1A astrocytes. Arrows are showing discreet band. (E) normalized fluo-4 fluorescence comparing vehicle (aCSF) and 100 nM TCAP-1 treated cells ( n = 3 for each treatment, where each n is an average of five cells per coverslip). Mean (± SEM) values from each group were submitted to two-way ANOVA and Bonferroni post hoc test. ** p < 0.01; *** p < 0.001.

    Techniques Used: In Vitro, Activation Assay, Gene Expression, Reverse Transcription Polymerase Chain Reaction, Positive Control, Fluorescence

    Related Articles

    In Vitro:

    Article Title: A Putative Role of Teneurin-2 and Its Related Proteins in Astrocytes
    Article Snippet: We searched for an astrocyte cell lineage that expresses teneurin gene expression in order to adopt it in future in vitro assays.We searched for an astrocyte cell lineage that expresses teneurin gene expression in order to adopt it in future in vitro assays.. For this, C8D1A mouse cerebellar immortalized astrocytes (#CRL-2541, ATCC, VA, USA) were used.. In addition, based on the fact that TCAP-1 is a bioactive peptide in neurons supported by in vitro and in vivo studies, we tested whether TCAP-1 is able to change calcium signaling in this cell lineage.In addition, based on the fact that TCAP-1 is a bioactive peptide in neurons supported by in vitro and in vivo studies, we tested whether TCAP-1 is able to change calcium signaling in this cell lineage.

    Activation Assay:

    Article Title: A Putative Role of Teneurin-2 and Its Related Proteins in Astrocytes
    Article Snippet: We searched for an astrocyte cell lineage that expresses teneurin gene expression in order to adopt it in future in vitro assays.We searched for an astrocyte cell lineage that expresses teneurin gene expression in order to adopt it in future in vitro assays.. For this, C8D1A mouse cerebellar immortalized astrocytes (#CRL-2541, ATCC, VA, USA) were used.. In addition, based on the fact that TCAP-1 is a bioactive peptide in neurons supported by in vitro and in vivo studies, we tested whether TCAP-1 is able to change calcium signaling in this cell lineage.In addition, based on the fact that TCAP-1 is a bioactive peptide in neurons supported by in vitro and in vivo studies, we tested whether TCAP-1 is able to change calcium signaling in this cell lineage.

    Gene Expression:

    Article Title: A Putative Role of Teneurin-2 and Its Related Proteins in Astrocytes
    Article Snippet: We searched for an astrocyte cell lineage that expresses teneurin gene expression in order to adopt it in future in vitro assays.We searched for an astrocyte cell lineage that expresses teneurin gene expression in order to adopt it in future in vitro assays.. For this, C8D1A mouse cerebellar immortalized astrocytes (#CRL-2541, ATCC, VA, USA) were used.. In addition, based on the fact that TCAP-1 is a bioactive peptide in neurons supported by in vitro and in vivo studies, we tested whether TCAP-1 is able to change calcium signaling in this cell lineage.In addition, based on the fact that TCAP-1 is a bioactive peptide in neurons supported by in vitro and in vivo studies, we tested whether TCAP-1 is able to change calcium signaling in this cell lineage.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: A Putative Role of Teneurin-2 and Its Related Proteins in Astrocytes
    Article Snippet: We searched for an astrocyte cell lineage that expresses teneurin gene expression in order to adopt it in future in vitro assays.We searched for an astrocyte cell lineage that expresses teneurin gene expression in order to adopt it in future in vitro assays.. For this, C8D1A mouse cerebellar immortalized astrocytes (#CRL-2541, ATCC, VA, USA) were used.. In addition, based on the fact that TCAP-1 is a bioactive peptide in neurons supported by in vitro and in vivo studies, we tested whether TCAP-1 is able to change calcium signaling in this cell lineage.In addition, based on the fact that TCAP-1 is a bioactive peptide in neurons supported by in vitro and in vivo studies, we tested whether TCAP-1 is able to change calcium signaling in this cell lineage.

    Positive Control:

    Article Title: A Putative Role of Teneurin-2 and Its Related Proteins in Astrocytes
    Article Snippet: We searched for an astrocyte cell lineage that expresses teneurin gene expression in order to adopt it in future in vitro assays.We searched for an astrocyte cell lineage that expresses teneurin gene expression in order to adopt it in future in vitro assays.. For this, C8D1A mouse cerebellar immortalized astrocytes (#CRL-2541, ATCC, VA, USA) were used.. In addition, based on the fact that TCAP-1 is a bioactive peptide in neurons supported by in vitro and in vivo studies, we tested whether TCAP-1 is able to change calcium signaling in this cell lineage.In addition, based on the fact that TCAP-1 is a bioactive peptide in neurons supported by in vitro and in vivo studies, we tested whether TCAP-1 is able to change calcium signaling in this cell lineage.

    Fluorescence:

    Article Title: A Putative Role of Teneurin-2 and Its Related Proteins in Astrocytes
    Article Snippet: We searched for an astrocyte cell lineage that expresses teneurin gene expression in order to adopt it in future in vitro assays.We searched for an astrocyte cell lineage that expresses teneurin gene expression in order to adopt it in future in vitro assays.. For this, C8D1A mouse cerebellar immortalized astrocytes (#CRL-2541, ATCC, VA, USA) were used.. In addition, based on the fact that TCAP-1 is a bioactive peptide in neurons supported by in vitro and in vivo studies, we tested whether TCAP-1 is able to change calcium signaling in this cell lineage.In addition, based on the fact that TCAP-1 is a bioactive peptide in neurons supported by in vitro and in vivo studies, we tested whether TCAP-1 is able to change calcium signaling in this cell lineage.



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    c8d1a mouse cerebellar immortalized astrocytes  (ATCC)
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    ATCC c8d1a mouse cerebellar immortalized astrocytes
    In vitro characterization of Ten/TCAP gene expressions and TCAP-1 induced activation in mouse cerebellar astrocytes. Gene expression was determined using RT-PCR with RNA extracted from <t>C8D1A</t> mouse cerebellar astrocytes. (A) teneurins 1, 3 and 4 were expressed ( n = 4) and teneurin 2 was not expressed ( n = 4). TCAPs 1-4 were expressed (TCAP-1, n = 3; TCAP-2, n = 5; TCAP-3, n = 3; TCAP-4, n = 3). β-actin served as positive control ( n = 5). (B) fluo-4 fluorescence shown prior to 100 nM TCAP-1 administration (non-stimulated). (C) fluo-4 fluorescence shown 3 min after 100 nM TCAP-1 administration (TCAP-1 treatment). (D) differential interference contrast image (DIC) C8D1A astrocytes. Arrows are showing discreet band. (E) normalized fluo-4 fluorescence comparing vehicle (aCSF) and 100 nM TCAP-1 treated cells ( n = 3 for each treatment, where each n is an average of five cells per coverslip). Mean (± SEM) values from each group were submitted to two-way ANOVA and Bonferroni post hoc test. ** p < 0.01; *** p < 0.001.
    C8d1a Mouse Cerebellar Immortalized Astrocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c8d1a+mouse+cerebellar+immortalized+astrocytes/C8-D1A/pmc06609321-143-2-8
    Average 96 stars, based on 1 article reviews
    c8d1a mouse cerebellar immortalized astrocytes - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    In vitro characterization of Ten/TCAP gene expressions and TCAP-1 induced activation in mouse cerebellar astrocytes. Gene expression was determined using RT-PCR with RNA extracted from C8D1A mouse cerebellar astrocytes. (A) teneurins 1, 3 and 4 were expressed ( n = 4) and teneurin 2 was not expressed ( n = 4). TCAPs 1-4 were expressed (TCAP-1, n = 3; TCAP-2, n = 5; TCAP-3, n = 3; TCAP-4, n = 3). β-actin served as positive control ( n = 5). (B) fluo-4 fluorescence shown prior to 100 nM TCAP-1 administration (non-stimulated). (C) fluo-4 fluorescence shown 3 min after 100 nM TCAP-1 administration (TCAP-1 treatment). (D) differential interference contrast image (DIC) C8D1A astrocytes. Arrows are showing discreet band. (E) normalized fluo-4 fluorescence comparing vehicle (aCSF) and 100 nM TCAP-1 treated cells ( n = 3 for each treatment, where each n is an average of five cells per coverslip). Mean (± SEM) values from each group were submitted to two-way ANOVA and Bonferroni post hoc test. ** p < 0.01; *** p < 0.001.

    Journal: Frontiers in Neuroscience

    Article Title: A Putative Role of Teneurin-2 and Its Related Proteins in Astrocytes

    doi: 10.3389/fnins.2019.00655

    Figure Lengend Snippet: In vitro characterization of Ten/TCAP gene expressions and TCAP-1 induced activation in mouse cerebellar astrocytes. Gene expression was determined using RT-PCR with RNA extracted from C8D1A mouse cerebellar astrocytes. (A) teneurins 1, 3 and 4 were expressed ( n = 4) and teneurin 2 was not expressed ( n = 4). TCAPs 1-4 were expressed (TCAP-1, n = 3; TCAP-2, n = 5; TCAP-3, n = 3; TCAP-4, n = 3). β-actin served as positive control ( n = 5). (B) fluo-4 fluorescence shown prior to 100 nM TCAP-1 administration (non-stimulated). (C) fluo-4 fluorescence shown 3 min after 100 nM TCAP-1 administration (TCAP-1 treatment). (D) differential interference contrast image (DIC) C8D1A astrocytes. Arrows are showing discreet band. (E) normalized fluo-4 fluorescence comparing vehicle (aCSF) and 100 nM TCAP-1 treated cells ( n = 3 for each treatment, where each n is an average of five cells per coverslip). Mean (± SEM) values from each group were submitted to two-way ANOVA and Bonferroni post hoc test. ** p < 0.01; *** p < 0.001.

    Article Snippet: For this, C8D1A mouse cerebellar immortalized astrocytes (#CRL-2541, ATCC, VA, USA) were used.

    Techniques: In Vitro, Activation Assay, Gene Expression, Reverse Transcription Polymerase Chain Reaction, Positive Control, Fluorescence